rabbit polyclonal antibody to sstr2  (Novus Biologicals)

Journal: Neuro-Oncology Advances

Article Title: Cytotoxicity on low-grade canine meningioma with the use of somatostatin analog (octreotide): An in vitro study

doi: 10.1093/noajnl/vdae111

Figure Lengend Snippet: Primary cell cultures from canine meningioma. (A) Cultured cells tend to arrange in fusiform clumps. May-Grunwald-Giemsa stain. (B) Many neoplastic cells show cytoplasmic or membrane EMA immunoreaction (Immunocytochemistry). (C) Diffuse marked cytoplasmic SSTR2 immunoreaction of the cultured neoplastic meningeal cells (Immunocytochemistry).

Article Snippet: In addition, rabbit anti-rat SSTR2 polyclonal antibody (1:500; 4°C overnight; Alomone Laboratories, Jerusalem, Israel) was used as a primary antibody.

Techniques: Cell Culture, Giemsa Stain, Membrane, Immunocytochemistry

Journal: Frontiers in Endocrinology

Article Title: Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue

doi: 10.3389/fendo.2020.00313

Figure Lengend Snippet: Characterization of PA tissue samples and pituispheres for hormone and cell marker expression by immunohistochemistry (IHC) and immunofluorescent analysis (IF).

Article Snippet: The following antibodies were used: mouse anti-Growth Hormone (GH) Antibody (MA5-11926), mouse anti-Prolactin (PRL) Monoclonal Antibody (MA5-11998), mouse anti-ACTH Monoclonal Antibody (MA5-13455), mouse anti-Thyroid Stimulating Hormone (TSHb) Antibody (MA5-12159), mouse anti-Luteinizing Hormone (LHb) Monoclonal Antibody (MA5-12138), mouse anti-Follicle Stimulating Hormone (FSHb) Monoclonal Antibody (MA5-12144), mouse anti-Follicle Stimulating Hormone alpha (CGA) Monoclonal Antibody (MA1-82895), rabbit anti-SSTR2 Polyclonal Antibody (PA3-109), rabbit anti-SSTR5 Polyclonal Antibody (PA3-112), rabbit anti-AIP Polyclonal Antibody (PA5-29862), mouse anti-Cytokeratin 8 (CK8) Monoclonal Antibody (MA5-14428) from Thermo Fisher Scientific, USA, and synonym of Tpit—rabbit anti-TBX19 Antibody (HPA072686) from Atlas Antibodies, Sweden.

Techniques: Marker, Expressing, Immunohistochemistry

Journal: World Journal of Surgical Oncology

Article Title: A new pathological classification of intrahepatic cholangiocarcinoma according to protein expression of SSTR2 and Bcl2

doi: 10.1186/s12957-021-02216-3

Figure Lengend Snippet: Immunostaining of IHCC tumor tissue for SSTR2 and Bcl2. a Expression of SSTR2 was only observed in the cell membrane of the cancer cells (× 200 magnification). b Expression of Bcl2 was observed in the cytoplasm of the cancer cells (× 200 magnification). c Expression of SSTR2 was observed in the normal large bile duct (× 200 magnification). d Expression of Bcl2 was observed in the normal small bile duct including bile ductule (× 200 magnification). IHCC: intrahepatic cholangiocarcinoma, SSTR2: somatostatin receptor 2, Bcl2: b cell leukemia/lymphoma 2

Article Snippet: The sections were incubated with a primary rabbit polyclonal antibody to SSTR2 (NB300-157, diluted 1:200 in PBS; NOVUS Biologicals LLC, Centennial, CO, USA) and a primary mouse monoclonal antibody to Bcl2 (M088701, diluted 1:40 in PBS; Dako, Santa Clara, CA, USA) overnight at 4 °C, respectively.

Techniques: Immunostaining, Expressing, Membrane

Journal: World Journal of Surgical Oncology

Article Title: A new pathological classification of intrahepatic cholangiocarcinoma according to protein expression of SSTR2 and Bcl2

doi: 10.1186/s12957-021-02216-3

Figure Lengend Snippet: Comparison of various factors among three groups of IHCC patients classified by SSTR2 and Bcl2 expression

Article Snippet: The sections were incubated with a primary rabbit polyclonal antibody to SSTR2 (NB300-157, diluted 1:200 in PBS; NOVUS Biologicals LLC, Centennial, CO, USA) and a primary mouse monoclonal antibody to Bcl2 (M088701, diluted 1:40 in PBS; Dako, Santa Clara, CA, USA) overnight at 4 °C, respectively.

Techniques: Comparison, Virus, Infection

Journal: World Journal of Surgical Oncology

Article Title: A new pathological classification of intrahepatic cholangiocarcinoma according to protein expression of SSTR2 and Bcl2

doi: 10.1186/s12957-021-02216-3

Figure Lengend Snippet: Overall survival and disease-free survival of IHCC patients classified by histological type. a Overall survival curves of IHCC patients. The survival of patients in the p-Group P was better than that of those in both the p-Group H and the p-Group U ( p = 0.098, p < 0.05, respectively). b Disease-free survival curves of IHCC patients. The survival of patients in the p-Group P was significantly better than that of those in both the p-Group H and the p-Group U ( p < 0.05). IHCC: intrahepatic cholangiocarcinoma, p-Group P: SSTR2 negative and Bcl2 positive, p-Group H: SSTR2 positive and Bcl2 negative, p-Group U: SSTR2 positive and Bcl2 positive or SSTR2 negative and Bcl2 negative

Article Snippet: The sections were incubated with a primary rabbit polyclonal antibody to SSTR2 (NB300-157, diluted 1:200 in PBS; NOVUS Biologicals LLC, Centennial, CO, USA) and a primary mouse monoclonal antibody to Bcl2 (M088701, diluted 1:40 in PBS; Dako, Santa Clara, CA, USA) overnight at 4 °C, respectively.

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: A new pathological classification of intrahepatic cholangiocarcinoma according to protein expression of SSTR2 and Bcl2

doi: 10.1186/s12957-021-02216-3

Figure Lengend Snippet: Comparison of various factors among two groups of grossly peripheral IHCC patients classified by SSTR2 and Bcl2 expression

Article Snippet: The sections were incubated with a primary rabbit polyclonal antibody to SSTR2 (NB300-157, diluted 1:200 in PBS; NOVUS Biologicals LLC, Centennial, CO, USA) and a primary mouse monoclonal antibody to Bcl2 (M088701, diluted 1:40 in PBS; Dako, Santa Clara, CA, USA) overnight at 4 °C, respectively.

Techniques: Comparison, Virus, Infection

Journal: World Journal of Surgical Oncology

Article Title: A new pathological classification of intrahepatic cholangiocarcinoma according to protein expression of SSTR2 and Bcl2

doi: 10.1186/s12957-021-02216-3

Figure Lengend Snippet: Overall survival and disease-free survival of grossly peripheral IHCC patients classified by histological type. a Overall survival curves of grossly peripheral IHCC patients. The survival of patients in the p-Group P was better than that of those in the p-Group H ( p = 0.150). b Disease-free survival curves of grossly peripheral IHCC patients. The survival of patients in the p-Group P was significantly better than that of those in the p-Group H ( p < 0.05). IHCC: intrahepatic cholangiocarcinoma, p-Group P: SSTR2 negative and Bcl2 positive, p-Group H; SSTR2 positive and Bcl2 negative

Article Snippet: The sections were incubated with a primary rabbit polyclonal antibody to SSTR2 (NB300-157, diluted 1:200 in PBS; NOVUS Biologicals LLC, Centennial, CO, USA) and a primary mouse monoclonal antibody to Bcl2 (M088701, diluted 1:40 in PBS; Dako, Santa Clara, CA, USA) overnight at 4 °C, respectively.

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: A new pathological classification of intrahepatic cholangiocarcinoma according to protein expression of SSTR2 and Bcl2

doi: 10.1186/s12957-021-02216-3

Figure Lengend Snippet: Representative images of gross and histological findings in IHCC tumors. a – c CT images showed the presence of a 5.5 cm, low density mass in the S3 segment of the liver in all phases. d The resected specimen showed the presence of a nodular tumor in the S3 segment of the liver. This tumor was consistent with the mass-forming type. e Histologic findings (× 100 magnification) revealed that the tumor consisted of duct-forming, abundant fibrotic stroma. The cancer cells had a clear cytoplasm. f SSTR2 expression can be seen in the cell membrane of IHCC tumor cells (× 100 magnification). g IHCC cancer cells were negative for Bcl2 (× 100 magnification). IHCC: intrahepatic cholangiocarcinoma, CT: computed tomography. SSTR2: somatostatin receptor 2, Bcl2: b-cell leukemia/lymphoma 2

Article Snippet: The sections were incubated with a primary rabbit polyclonal antibody to SSTR2 (NB300-157, diluted 1:200 in PBS; NOVUS Biologicals LLC, Centennial, CO, USA) and a primary mouse monoclonal antibody to Bcl2 (M088701, diluted 1:40 in PBS; Dako, Santa Clara, CA, USA) overnight at 4 °C, respectively.

Techniques: Expressing, Membrane, Computed Tomography

Journal: World Journal of Surgical Oncology

Article Title: A new pathological classification of intrahepatic cholangiocarcinoma according to protein expression of SSTR2 and Bcl2

doi: 10.1186/s12957-021-02216-3

Figure Lengend Snippet: Analysis of overall survival in IHCC patients

Article Snippet: The sections were incubated with a primary rabbit polyclonal antibody to SSTR2 (NB300-157, diluted 1:200 in PBS; NOVUS Biologicals LLC, Centennial, CO, USA) and a primary mouse monoclonal antibody to Bcl2 (M088701, diluted 1:40 in PBS; Dako, Santa Clara, CA, USA) overnight at 4 °C, respectively.

Techniques: Expressing

Journal: EJNMMI Research

Article Title: Detection and therapy of neuroblastoma minimal residual disease using [ 64/67 Cu]Cu-SARTATE in a preclinical model of hepatic metastases

doi: 10.1186/s13550-021-00763-0

Figure Lengend Snippet: Images of tumor histology and autoradiography data showing distribution of 64 Cu-SARTATE in tumor deposits and surrounding liver. Top row a shows a hematoxylin and eosin-stained frozen section of liver tissue (pink) containing darker purple tumor deposits and its corresponding autoradiograph ( b ) (size scale bar bottom left of a , autoradiography data scale bar right of b ). Details of regions indicated by lower case a–d are shown in figure. Middle row c shows a contiguous section that has been stained using immunohistochemistry to demonstrate SSTR2 expression (dark brown) counter-stained with hematoxylin (blue) and its autoradiograph ( d ). Uptake of the radiolabeled peptide was highest in regions of tumor that were both viable and stained strongly for the target SSTR2. Bottom row ( e ) shows a surface plot of the autoradiograph B generated in ImageJ showing the relative uptakes in liver, viable SSTR2-positive tumor and necrotic tumor. Uptake in the small group of cells surrounded by liver “c” and the small SSTR2-positive deposit “d” are indicated with arrows for orientation

Article Snippet: They were then stained using either hematoxylin and eosin to demonstrate general morphology or immunohistochemistry (IHC) to demonstrate SSTR2 expression (rabbit anti-SSTR2 polyclonal antibody: Novus Biologicals; Centennial, CO).

Techniques: Autoradiography, Staining, Immunohistochemistry, Expressing, Generated

Journal: EJNMMI Research

Article Title: Detection and therapy of neuroblastoma minimal residual disease using [ 64/67 Cu]Cu-SARTATE in a preclinical model of hepatic metastases

doi: 10.1186/s13550-021-00763-0

Figure Lengend Snippet: Detail from Fig. . Radionuclide distribution was heterogeneous in the viable tumor to the left of region a , and in the small deposit to the right with low uptake in the surrounding liver. There was high uptake in a small, viable deposit to the right of region b but very low uptake in the large, necrotic mass to the left. In region c , there was high uptake in an isolated group of cells (40 × magnification), and its corresponding autoradiograph. Images of the same region of cells (middle row, right and far right, photographed at 100 × and 200 × magnification) demonstrate the ability of 64 Cu-SARTATE to target small tumor deposits. Region d refers to a tumor deposit that stained strongly for SSTR2 by IHC, then the corresponding autoradiograph showing high uptake in the tumor and low uptake in the surrounding liver. The next image is a × 200 magnification photograph of the same deposit and finally (right) the primary omission control of the same region from a contiguous tissue section confirming specificity of staining

Article Snippet: They were then stained using either hematoxylin and eosin to demonstrate general morphology or immunohistochemistry (IHC) to demonstrate SSTR2 expression (rabbit anti-SSTR2 polyclonal antibody: Novus Biologicals; Centennial, CO).

Techniques: Isolation, Autoradiography, Staining, Control

Journal: Acta Pharmacologica Sinica

Article Title: Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury

doi: 10.1038/aps.2017.159

Figure Lengend Snippet: The expression levels of the main SSTRs in rat tissues, and the influence of SSTRs on the distribution of OCT in rats. (A) The relative expression levels of SSTR2, SSTR3 and SSTR5 in rat stomach, duodenum, jejunum, ileum and colon; (B) The expression of SSTR2 in rat stomach sections; (C) The expression of SSTR3 in rat stomach sections as measured by fluorescence immunoassay; (D) The expression of SSTR5 in rat stomach sections; (E) The effects of SSTR2 antagonists on the distribution of OCT. * P<0.05.

Article Snippet: After washing with phosphate-buffered solution (PBS, pH 7.4) three times, the stomach slices were blocked with 10% fetal calf serum (FCS) for 30 min. Polyclonal rabbit anti-mouse SSTR2, SSTR3 and SSTR5 primary antibodies (Alomone Lab, Jerusalem, Israel) were used at dilutions of 1:500, 1:250 and 1:250, respectively.

Techniques: Expressing, Fluorescence

Journal: Acta Pharmacologica Sinica

Article Title: Pharmacokinetic and pharmacodynamic evidence for developing an oral formulation of octreotide against gastric mucosal injury

doi: 10.1038/aps.2017.159

Figure Lengend Snippet: Protective mechanism of OCT in WIRS animals. (A) The effects of OCT on the expression of SST. * P<0.05; (B) The effects of OCT on the expression of gastrin; (C) The effects of OCT on the expression of SSTR2. * P<0.05 vs normal group. # P<0.05 vs model group; (D) A pyloric ligation-induced ulcer model; (E) The lesion areas (%) of rat stomachs. * P<0.05; (F) Gastrin levels. ** P<0.01 vs normal group. # P<0.05 vs model group; (G) Gastric acid pH levels. ** P<0.01.

Article Snippet: After washing with phosphate-buffered solution (PBS, pH 7.4) three times, the stomach slices were blocked with 10% fetal calf serum (FCS) for 30 min. Polyclonal rabbit anti-mouse SSTR2, SSTR3 and SSTR5 primary antibodies (Alomone Lab, Jerusalem, Israel) were used at dilutions of 1:500, 1:250 and 1:250, respectively.

Techniques: Expressing, Ligation

Journal: PloS one

Article Title: Loss of somatostatin receptor subtype 2 in prostate cancer is linked to an aggressive cancer phenotype, high tumor cell proliferation and predicts early metastatic and biochemical relapse.

doi: 10.1371/journal.pone.0100469

Figure Lengend Snippet: Figure 1. SSTR2 immunohistochemistry in normal prostate tissue and prostate cancer. Microphotographs of tissue microarray cores showing normal prostate and prostate cancer tissues: SSTR2-positive normal epithelium (A), SSTR2-negative (B) and SSTR2-positive prostate cancer tissue (C) as well as SSTR2-negative cancer cells next to strongly SSTR2-positive normal epithelial cells (D). doi:10.1371/journal.pone.0100469.g001

Article Snippet: TMA sections were de-paraffinized followed by heatinduced antigen retrieval in an autoclave in acetate buffer pH 6.0 for 5 min. Primary polyclonal rabbit anti-SSTR2 antibody (HPA007264, Atlas Antibodies, Stockholm, Sweden) was used in a final dilution of 1:150.

Techniques: Immunohistochemistry, Microarray

Journal: PloS one

Article Title: Loss of somatostatin receptor subtype 2 in prostate cancer is linked to an aggressive cancer phenotype, high tumor cell proliferation and predicts early metastatic and biochemical relapse.

doi: 10.1371/journal.pone.0100469

Figure Lengend Snippet: Figure 5. Clinical impact of SSTR2 staining on event-free survival. PSA recurrence-free survival gradually declines from strong staining of cancer spots over moderate and weak to SSTR2-negative prostate cancers (A, p = 0.0009, Kaplan-Meier analysis with Log-Rank test). Prostatectomized patients with SSTR2-negative prostate cancers also have impaired metastasis-free survival (B, p = 0.0452, Kaplan-Meier analysis with Log-Rank test). doi:10.1371/journal.pone.0100469.g005

Article Snippet: TMA sections were de-paraffinized followed by heatinduced antigen retrieval in an autoclave in acetate buffer pH 6.0 for 5 min. Primary polyclonal rabbit anti-SSTR2 antibody (HPA007264, Atlas Antibodies, Stockholm, Sweden) was used in a final dilution of 1:150.

Techniques: Staining